ABSTRACT
OBJECTIVE: To analyze the production of antibodies to polysaccharide antigens in patients with ataxia-telangiectasia.PATIENTS AND METHODS: We used the ELISA technique to measure the levels of IgG antibodies to serotypes 1, 3, 5, 6B, 9V and 14 of Streptococcus pneumoniae in 14 patients with ataxia-telangiectasia before and after immunization with 23-valent polysaccharide vaccine. Adequate response to individual polysaccharide can be defined as a postimmunization antibody titer equal to or greater than 1.3 µg/ml or as a minimum fourfold increase over the baseline (preimmunization) value. RESULTS: Six (43%) patients showed an absent response to all serotypes analyzed. Four patients showed adequate response to only one serotype, one patient to two serotypes, two patients to three serotypes and only one patient to four out of six serotypes analyzed. No patient had adequate response to all serotypes tested. Postimmunization pneumococcus IgG levels were higher than preimmunization levels to all serotypes analyzed, except for serotype 3. In spite of this, the mean postimmunization levels were lower than 1.3 µg/ml in all serotypes, except for serotype 14. Mean increment was less than four in all serotypes analyzed. CONCLUSION: Our results suggest that patients with ataxia-telangiectasia are at a high risk of having an impaired response to pneumococcus, which may be one of the causes of recurrent sinopulmonary infections in these patients.
Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Antibodies, Bacterial/blood , Ataxia Telangiectasia/immunology , Polysaccharides, Bacterial/immunology , Pneumococcal Vaccines/immunology , Enzyme-Linked Immunosorbent Assay , Immunoglobulin G/blood , Pneumococcal Infections/immunology , VaccinationABSTRACT
E. Coli es uno de los gérmenes más frecuentemente aislado en sepsis y meningitis neonatal. La susceptibilidad a este germen se atribuye a la falta de anticuerpos protectores. El objetivo del presente trabajo fue evaluar el pasaje transplacentário de anticuerpos dirigido contra muestras de suero de madres y sus respectivos recién nacidos. El análisis se realizó por el método de focalización isoeléctrica asociada a transferencia de afinidad (affinity blotting). Observamos espectrotipos idénticos de IgG y subclases de IgG1 e Igg2 anti-E. coli en las muestras de madre y sus respectivos recién nacidos. Demostramos así el pasaje transplacentaário de anticuerpos específicos contra este germen
Subject(s)
Infant, Newborn , Meningitis , Infant, Newborn , SepsisABSTRACT
Com objetivo de verificar o comportamento imunologico do recem-nascido (RN) frente a um agravo infeccioso, estudamos 60 RN que apresentavam fatores de risco para infeccao precoce (ruptura prematura de membranas, amnionite clinica, ou infeccao do trato urinario) do ponto de vista infeccioso e imunologico. Todos foram classificados em tres grupos de idade gestacional : inferior a 34 semanas, entre 34 e 36 6/7 semanas e > 37 semanas. O diagnostico de sepse foi realizado atraves de criterios clinicos e laboratorias, incluindo-se entre os exames as dosagens de imunoglobulinas (IgG, IgM, IgA) e complemento total (CH50), obtidos do RN ao nascimento e no quinto dia de vida...